PCR

A PCR (Polymerase Chain Reaction) technika segítségével egyszerűen és hatékonyan amplifikálhatóak a nukleinsavak. A Bioline cég számos PCR és RT-PCR enzimet kínál, és segítséget nyújt abban is, hogy mindenki a legoptimálisabb enzimet illetve kitet válassza ki annak megfelelően, hogy milyen alkalmazásokhoz kívánja használni a PCR terméket (klónozás, szekvenálás, jelölt próbák). A siker záloga, hogy mindig a legmegfelelőbb enzimet illetve kitet válasszuk.

További részletek a Bioline weboldalon

PCR enzimek specifikációs táblázata

Specifikációk:
Általános reagensek PCR munkához
Rutin PCR
Hot start PCR
Nagy hűségű PCR
Hosszú templátok
GC gazdag templátok
PCR genotipizálás
PCR mixek
Kontroll templátok

Általános reagensek PCR munkához

Nukleotidok

Pufferek, egyéb PCR reagensek

Nukleotidok

*INDIVIDUALS*
BIO39032	dITP, 100mM	2'-Deoxyinosine 5' Triphosphate	25µmol, (1 x 250µl)
BIO39035	dUTP, 100mM	2'-Deoxyuridine 5'Triphosphate	25µmol, (1 x 250µl)
BIO39036	dATP, 100mM	2' Deoxyadenosine 5'-Triphosphate	25µmol, (1 x 250µl)
BIO39037	dGTP, 100mM	2'-Deoxyguanosine 5' -Triphosphate	25µmol, (1 x 250µl)
BIO39038	dCTP, 100mM	2'-Deoxycytidine 5'- Triphosphate	25µmol, (1 x 250µl)
BIO39039	dTTP, 100mM	2'-Deoxythymidine 5'-Triphosphate	25µmol, (1 x 250µl)

BIO39046	Hydroxymethylated dCTP, 100mM	5 Hydroxymethyl 2'-Deoxycytidine 5'- Triphosphate	25µmol, (1 x 250µl)

BIO39047	Aminoally dUTP	Can be incorporated into DNA by polymerases such as Taq, Klenow fragment and by Reverse Transcriptases	5µmol, (1 x 50µl)
BIO39048	Aminoally dUTP	Can be incorporated into DNA by polymerases such as Taq, Klenow fragment and by Reverse Transcriptases	10µmol, (1 x 100µl)
*SETS*
BIO39025	dNTP Set, 100mM	Set of ready-to-use molecular grade dNTP solutions	4 x 25µmol, (4 x 250µl)
BIO39026	dNTP Set, 100mM	Set of ready-to-use molecular grade dNTP solutions	4 x 100µmol, (4 x 4 x 250µl)
BIO39049	dNTP Set, 100mM	Set of ready-to-use molecular grade dNTP solutions	4 x 100µmol, (4 x 1ml)
BIO39027	dNTP Set, 100mM	Set of ready-to-use molecular grade dNTP solutions	4 x 500µmol, (4 x 20 x 250µl)
*MIXES*
BIO39044	dNTP Mix, 10mM Final Conc.	Ready-to-go molecular grade dNTP Mix	10µmol, (1ml vol.)
BIO39053	dNTP Mix, 10mM Final Conc.	Ready-to-go molecular grade dNTP Mix	100µmol, (10 x 1ml)
BIO39043	dNTP Mix, 40mM Final Conc.	Ready-to-go molecular grade dNTP Mix	20µmol, (1 x 500µl)
BIO39028	dNTP Mix, 100mM Final Conc.	Ready-to-go molecular grade dNTP Mix	50µmol, (1 x 500µl)
BIO39029	dNTP Mix, 100mM Final Conc.	Ready-to-go molecular grade dNTP Mix	200µmol, (4 x 500µl)

BIO39041	dUTP Mix, 50mM Final Conc.	Ready-to-go molecular grade dNTP Mix	25µmol, (1 x 500µl)
*RIBONUCLEOTIDES*
BIO39050	NTP Mix, 100mM Final Conc.	Ready=to-go molecular grade NTP Mix	100µmol, (1 x 1ml)
BIO39052	NTP Set, 100mM	Set of ready-to-go molecular grade NTP solutions	4 x 25µmol, (4 x 250µl)

Pufferek, egyéb PCR reagensek

Cikkszám	Termék	Leírás	Kiszerelés
BIO37025	NH4 Buffer, 10x		3 x 1.2ml
BIO37064	NH4 Buffer, 10x incl. 15mM MgCl2		3 x 1.2ml
BIO37026	MgCl2 Solution, 50mM		3 x 1.2ml
BIO37066	KCl Buffer, 10x		3 x 1.2ml
BIO37030	OptiBuffer, 10x	Non-Tris incubation buffer to improve the performance on any thermostable DNA polymerase	~ 200 Reactions, 1.2ml
BIO37031	OptiBuffer, 10x	Non-Tris incubation buffer to improve the performance on any thermostable DNA polymerase	~ 600 Reactions , 1.2ml x 3
BIO37101	MangoTaq Buffer 5x, Colourless		10ml
BIO37102	MangoTaq Buffer 5x, Coloured		10ml

BIO37032	Hi-Spec Additive, 5x	Additive for High-Specificity	3x1.2 ml
BIO37041	PolyMate Additive, 2x	Additive for High-Specificity and Yield	2 x 1.2ml

BIO37080	Water, 18.2MΩ PCR Grade	Molecular Grade	10 x 10ml

BIO37089	PCR Buffer A, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37090	PCR Buffer B, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37091	PCR Buffer C, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37092	PCR Buffer D, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37093	PCR Buffer E, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37094	PCR Buffer F, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml

BIO37095	PCR Buffer G, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37096	PCR Buffer H, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37097	PCR Buffer I, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37098	PCR Buffer J, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37099	PCR Buffer K, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml
BIO37100	PCR Buffer L, 2x	Ready-to-use Reaction buffer with proprietary mix of enhancers	2.5ml

Rutin PCR

általános, mindennapi rutin PCR munkára használható enzimek, kedvező áron

Cikkszám	Termék	Leírás	Kiszerelés
BIO21080	HyBripol DNA Polymerase	TAQ subsititute, economical	1000 Units
BIO21081	HyBripol DNA Polymerase	TAQ subsititute, economical	2500 Units
O21040	BIOTAQ DNA Polymerase	Regular TAQ	500 Units
BIO21060	BIOTAQ DNA Polymerase	Regular TAQ	2500 Units
BIO21071	BIOTAQ PCR Kit	All reagents for polymerase reactions	500 Units
BIO21038	BIOTAQ Red DNA Polymerase	Regular TAQ, Red	100 Units
BIO21061	BIOTAQ Red DNA Polymerase	Regular TAQ, Red	2500 Units
BIO21083	MangoTaq DNA Polymerase	Regular TAQ with 2 buffer systems, one with a two-dye reaction buffer and one colourless	1000 Units
BIO21082	MangoTaq DNA Polymerase	Regular TAQ with 2 buffer systems, one with a two-dye reaction buffer and one colourless	2000 Units
BIO21078	MangoTaq DNA Polymerase	Regular TAQ with 2 buffer systems, one with a two-dye reaction buffer and one colourless	5000 Units
BIO21079	MangoTaq DNA Polymerase	Regular TAQ with 2 buffer systems, one with a two-dye reaction buffer and one colourless	20,000 Units

Hot start PCR

„hot start” technológia – pontos, megbízható PCR

Cikkszám	Termék	Leírás	Kiszerelés
BIO21046	IMMOLASE DNA Polymerase	Hot-Start	250 Units
BIO21047	IMMOLASE DNA Polymerase	Hot-Start	500 Units
BIO21048	IMMOLASE DNA Polymerase	Hot-Start	5000 Units
BIO21088	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	250 Units
BIO21089	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	500 Units
BIO21090	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	2500 Units
BIO21068	AccuSure DNA Polymerase	Hot-start with high fidelity	250 Units
BIO21069	AccuSure DNA Polymerase	Hot-start with high fidelity	500 Units

Nagy hűségű PCR

proofreading enzimek, melyek nagy hűséggel másolják a DNS-t

Cikkszám	Termék	Leírás	Kiszerelés
BIO21051	ACCUZYME DNA Polymerase	High-Fidelity	250 Units
BIO21052	ACCUZYME DNA Polymerase	High-Fidelity	500 Units
BIO21091	ACCUZYME Red DNA Polymerase	High-Fidelity, with Red Dye	250 Units
BIO21092	ACCUZYME Red DNA Polymerase	High-Fidelity, with Red Dye	500 Units
BIO21101	pfu DNA Polymerase	High-Fidelity	250 Units
BIO21102	pfu DNA Polymerase	High-Fidelity	500 Units
BIO21068	AccuSure DNA Polymerase	Hot-start with high fidelity	250 Units
BIO21069	AccuSure DNA Polymerase	Hot-start with high fidelity	500 Units
BIO21049	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	250 Units
BIO21050	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	500 Units
BIO21064	BIO-X-ACT Short DNA Polymerase	Short-range, difficult templates	250 Units
BIO21065	BIO-X-ACT Short DNA Polymerase	Short-range, difficult templates	500 Units
BIO21088	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	250 Units
BIO21089	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	500 Units
BIO21090	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	2500 Units
BIO21098	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	250 Units
BIO21099	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	500 Units

Hosszú templátok

10 kb-nál nagyobb templátok esetén ajánlott az alkalmazásuk

Cikkszám	Termék	Leírás	Kiszerelés
BIO21049	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	250 Units
BIO21050	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	500 Units
BIO21098	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	250 Units
BIO21099	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	500 Units

GC gazdag templátok

GC gazdag templátok megfelelő amplifikálására kifejlesztett speciális polimerázok

Cikkszám	Termék	Leírás	Kiszerelés
BIO21058	Diamond DNA Polymerase	High Specificity suited to GC-Rich templates, SNP, mutation detection and MicroSatalite	250 Units
BIO21059	Diamond DNA Polymerase	High Specificity suited to GC-Rich templates, SNP, mutation detection and MicroSatalite	500 Units
BIO21098	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	250 Units
BIO21099	Velocity DNA Polymerase	High Speed, high fidelity DNA Polymerase	500 Units
BIO21049	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	250 Units
BIO21050	BIO-X-ACT Long DNA Polymerase	(original version) long-range, difficult templates	500 Units
BIO21064	BIO-X-ACT Short DNA Polymerase	Short-range, difficult templates	250 Units
BIO21065	BIO-X-ACT Short DNA Polymerase	Short-range, difficult templates	500 Units
BIO21088	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	250 Units
BIO21089	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	500 Units
BIO21090	SAHARA DNA Polymerase	Hot-Start for Short-range, difficult templates	2500 Units

PCR genotipizálás

speciálisan genotipizálásra kifejlesztett enzim, mely alkalmas az ismétlődő dinukleotid markerek amplifikálására

Cikkszám	Termék	Leírás	Kiszerelés
BIO21058	Diamond DNA Polymerase	High Specificity suited to GC-Rich templates, SNP, mutation detection and MicroSatalite	250 Units
BIO21059	Diamond DNA Polymerase	High Specificity suited to GC-Rich templates, SNP, mutation detection and MicroSatalite	500 Units

PCR mixek

Cikkszám	Termék	Leírás	Kiszerelés
BIO25011	BioMix, 2x	Ready-to-go pre-mix	100 Reactions
BIO25012	BioMix, 2x	Ready-to-go pre-mix	500 Reactions

BIO25005	BioMix Red, 2x	Ready-to-go pre-mix with red dye	100 Reactions
BIO25006	BioMix Red, 2x	Ready-to-go pre-mix with red dye	500 Reactions

BIO25019	ImmoMix, 2x	Heat-Activated, Ready-to-go pre-mix	100 Reactions
BIO25020	ImmoMix, 2x	Heat-Activated, Ready-to-go pre-mix	500 Reactions

BIO25021	ImmoMix Red, 2x	Heat-Activated, Ready-to-go pre-mix with red dye	100 Reactions
BIO25022	ImmoMix Red, 2x	Heat-Activated, Ready-to-go pre-mix with red dye	500 Reactions

BIO25023	BIO-X-ACT Long Mix, 2x	Long-range, difficult templates, Ready-to-go pre-mix	100 Reactions
BIO25024	BIO-X-ACT Long Mix, 2x	Long-range, difficult templates, Ready-to-go pre-mix	500 Reactions

BIO25025	BIO-X-ACT Short Mix, 2x	Short-range, difficult templates, Ready-to-go pre-mix	100 Reactions
BIO25026	BIO-X-ACT Short Mix, 2x	Short-range, difficult templates, Ready-to-go pre-mix	500 Reactions

BIO25027	ACCUZYME Mix, 2x	High Fidelity, Ready-to-go pre-mix	100 Reactions
BIO25028	ACCUZYME Mix, 2x	High Fidelity, Ready-to-go pre-mix	500 Reactions

BIO25029	AccuSure Mix, 2x	High Fidelity Heat-Activated, Ready-to-go pre-mix	100 Reactions
BIO25030	AccuSure Mix, 2x	High Fidelity Heat-Activated, Ready-to-go pre-mix	500 Reactions

BIO25031	Diamond Mix, 2x	High Specificity, Ready-to-go pre-mix	100 Reactions
BIO25032	Diamond Mix, 2x	High Specificity, Ready-to-go pre-mix	500 Reactions

BIO25033	MangoMix, 2x	Ready-to-go pre-mix with red and yellow dye	250 Reactions
BIO25034	MangoMix, 2x	Ready-to-go pre-mix with red and yellow dye	1000 Reactions

BIO25035	SAHARAMix, 2x	Ready-to-go pre-mix for Short Range	100 Reactions
BIO25036	SAHARAMix, 2x	Ready-to-go pre-mix for Short Range	500 Reactions

Kontroll templátok

Cikkszám	Termék	Leírás	Kiszerelés
BIO35025	Human Genomic DNA	Highly-purified genomic DNA for southern blotting, library construction and as a control template	500µl @ 200ng/µl
BIO35026	Rat Genomic DNA	Highly-purified genomic DNA for southern blotting, library construction and as a control template	500µl @ 200ng/µl
BIO35027	Mouse Genomic DNA	Highly-purified genomic DNA for southern blotting, library construction and as a control template	500µl @ 200ng/µl